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Date : Thursday, April 15, 2010
Time : 3:39 PM
Title : Advantages and Disadvantages of Chromatography


Advantages of Column Chromatography
Column chromatography is advantageous over most other chromatographic techniques because it can be used in both analytical and preparative applications. Not only can column chromatography be used to determine the number of components of a mixture, but it can also be used to separate and purify substantial quantities of those components for subsequent analysis. This is in contrast to paper chromatography, which is solely an analytical method.
For example, while paper chromatography is easily applied to see whether a purple coloured beverage contains a mixture of dyes, it is not practical to further analyze the separated dyes given the necessarily very small size of the initial sample. A preparative method like column chromatography allows you to do just that. Separating the purple food dye on an appropriately set up column with good technique will leave you with cleanly separated blue and red dyes in large enough amounts for further investigation. Thus, column chromatography should be used any time you want to separate a mixture of liquids or solutes into its components, and work with these components individually. In fact, it is the most frequently used method of purifying mixtures of products in research laboratories.
Disadvantages of Column Chromatography
With all its advantages and preparative power, column chromatography does have its complications. Properly setting up the column (something that will be done for you prior to experiment) requires some technical skill and manual dexterity, and takes some time. Column chromatography is less foolproof than paper chromatography and requires constant attention while the experiment is being performed: collection vessels must be frequently switched and solvent levels need to be topped up.In short, running a column is time-consuming and tedious, especially for large samples. If it is unnecessary to preparatively separate large quantities of sample, analytical methods such as paper chromatography may be more suitable and easier to perform.

Choo Jia Wei
Adapted From:http://www.chem.ubc.ca/courseware/154/tutorials/exp3A/columnchrom/