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Date : Sunday, April 18, 2010
Time : 7:54 PM
Title : CHROMATOGRAPHY

Yes because Chromatography is a process which can be used to isolate the various components of a mixture and has a broad of range of physical methods used to seperate and analyze complex mixtures.Chromatography is the science which is studies the separation of molecules based on differences in their structure and/or composition.The technique relies on the idea that the solvent and the paper both have an attraction for the components in a mixture. The solvent creeps along the surface of the paper. If a material is placed on one spot on the paper and is soluble in the liquid solvent, the material will be dissolved when the solvent moves over it. The material will move along with the solvent. Each compound in a mixture will have its own characteristic balance of attractions to solvent and to paper, so all will not move at the same speed. Eventually this difference in speed will separate the compounds.
In paper chromatography when the conditions are kept constant, a particular compound always travels a fixed percentage of the distance traveled by the solvent front.



Advantages:
Column chromatography is advantageous over most other chromatographic techniques because it can be used in both analytical and preparative applications.Not only can column chromatography be used to determine the number of components of a mixture, but it can also be used to separate and purify substantial quantities of those components for subsequent analysis. This is in contrast to paper chromatography, which is solely an analytical method.
This analytical method is quick to perform and easy to master.Another advantage of this method is that it requires a relatively small sample and is very inexpensive - a big plus in today's cost-conscious world.

Disadvantages:
With all its advantages and preparative power, column chromatography does have its complications. Properly setting up the column (something that will be done for you prior to experiment) requires some technical skill and manual dexterity, and takes some time. Column chromatography is less foolproof than paper chromatography and requires constant attention while the experiment is being performed: collection vessels must be frequently switched and solvent levels need to be topped up.
Some mixtures are very difficult to separate by paper chromatography; and any species that is not colored is difficult to observe on the chromatogram.


Types of chromatography:

gas chromatography
paper chromatography
liquid chromatography
thin layer chromatography
ion exchange chromatography
column chromatography


More info....
http://www.chemguide.co.uk/analysis/chromatography/paper.html
http://teaching.shu.ac.uk/hwb/chemistry/tutorials/chrom/gaschrm.htm



Video....
http://www.wonderhowto.com/how-to-perform-paper-chromatography-chemistry-lab-269382/



Amirul Haziq[22]